Table 3.

Sequence of oligonucleotides and parameters used for amplification of gene coding for ORF 3C6.

Protein	Restriction Enzyme (Sequence 5′−3′)	Oligonucleotides	MT*	Fragment size
ORF3C6	Foward_BsaI GGTCTCCCATGAGTGATCAATATCT		62,9 °C	897
	pb Reverse_HindIII AAGCTTTTAAGTCGAGTCCTGACCC		64,6 °C

*MT = Melting Temperature.