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. 2020 Jan 28;10:1356. doi: 10.1038/s41598-020-58023-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Time-resolved difference spectra and photocycling rate of HSG119. Absorption spectra (upper panel) were collected every 3 second after light illumination until 15 second. Clearly O intermediate was produced, but M intermediate was not detected. We measured rise and decay rate of ground and O intermediate states (bottom panel).