Table 1.

Primer sequences and amplicon characteristics of 15 candidate RGs and validation gene for RT-qPCR analysis, *gene known to have different levels of expression in C. pilosula was used to validate the candidate RGs.

Gene symbol	Gene description	Primer sequences (5′−3′)	Amplicon Size(bp)	TM (°C)	Efficiency (%)
CYP20-1	Cytochrome P450 20-1	GGACCAGACACCAATGGTTCAC	188	58.0	91.5
		AAGGGTAGCTCTCCGCTGTC
ABCC8	ABC-transporter C family member 8	AGCCCTAACGGGTACCCAAG	136	58.0	96.2
		TGGCCTCTTGTCCTCCACAA
ACTβ	Beta-Actin	AGAGAAAGCGCTGAAATGCCA	103	58.0	102.1
		AGCCTTGGGACGAAACCCTA
UPL-RHF2A	E3 ubiquitin-protein ligase RHF2A	GAGGGAGGGCTCAAGGAGTC	172	58.0	92.5
		AGCTGCAAATGGGACAGACG
EF1α	Elongation factor 1-alpha	GCCTGGTGACAACGTTGGAT	191	58.0	107.2
		GCGAGGTGTGGCAATCAAGA
EIF4A-14	Eukaryotic initiation factor 4A	GTGGCAGGGAAATCGGTTGT	187	58.0	98.1
		GCTGAATAGCGGAGGGCTTC
RPL5B	60 S ribosomal protein L5	CAGCTGCTTACGCCCATGAA	180	58.0	95
		CCGCCGGTTCAACAGAGAAA
RPL13	60 S ribosomal protein L13	AGGAACTTGCAACAGCCACA	176	58.0	93.7
		CGCAGCCCTCTTAAGCCTTT
G6PDH	glucose-6-phosphate dehydrogenase	TGGTGACGTAGTGCAGAGTC	187	58.0	91.3
		AGCATCAACGTTGTCCTCAGA
GAPDH	Glyceraldehyde 3-phosphate dehydrogenase	CCAAGAAGGCAGGCATGGTT	150	58.0	94.1
		CCTTTACGGCTGTGCACCTT
TUBα3	Alpha-tubulin	TGACTGGTGCCCTACTGGTT	178	58.0	106.4
		CGCCCTCTTTGCGTACATGA
PP2A57θ	Protein phosphatase 2A-57	CCTGCACTGGAGAGAAACGG	183	58.0	102.5
		ACGTTTCCATGCAGCTTCCC
PP2A59γ	Protein phosphatase 2A-59	GCGGACTCACTGGAACCAAG	166	58.0	95.6
		CAGGCGCTTCCAGGTTGATT
UBC18	Ubiquitin-conjugating enzyme 18	CCAGCTCCTCTACACCCTCA	152	58.0	104.7
		TCTTCTGGTCGTTGCTTCGC
UBCE32	Ubiquitin-conjugating enzyme 32	TGGTGACCAGGCAAACGATG	175	58.0	91.8
		CCTCAAGACTGCAGGAGTGG
UGPase*	UDP-glucose pyrophosphorylase	TGATGGCTATGTGACCCGGAA	187	58.0	102.1
		ACCCTTGAGAACGACGGAAGA