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. 2020 Jan 28;10:1283. doi: 10.1038/s41598-020-58239-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Confocal images of HepG2 cells incubated with 30 μM of I₂-IR783-Mpip for 6 h and colocalization of I₂-IR783-Mpip with LysoTracker green (Pearson’s R value = 0.68 in pH 5.0 and 0.68 in pH 7.4) and MitoTracker Green (Pearson’s R value = 0.31 in pH 5.0 and 0.45 in pH 7.4). (B) Flow cytometry of HepG2 cells incubated with 15 μM of I₂-IR783-Mpip for 12 h in culture media pH 5.0 and 7.4. (C) Flow cytometry of HepG2 and HEK293 cells incubated with 15 μM of I₂-IR783-Mpip for 12 h in culture media pH 5.0 and 7.4. Scale bars = 20 μm.