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. 2020 Jan 28;10:1349. doi: 10.1038/s41598-020-58452-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Apelin expression and distribution during ischemia-reperfusion (I/R) injury. (A) Quantification of apelin mRNA levels in the I/R site from the beginning of ischemia to 72 hours after reperfusion by quantitative reverse transcriptase–PCR (RT–PCR). The end of ischemia was assigned 0 hour. Data are relative to mRNA level in -12 hours. Values were determined in n = 4. (B) Expression and distribution of apelin in the skin before ischemia (control) and at 4 hours after reperfusion (red: CD31, green: apelin). Scale bar = 20 μm. All values represent mean ± SEM. **P < 0.01, *P < 0.05.