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. 2020 Jan 28;10:1349. doi: 10.1038/s41598-020-58452-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Apelin reduced oxidative stress induced by cutaneous I/R injury in vivo. (A) Representative image of luminescence signals in cutaneous I/R area in OKD48 mice at 24 hours after reperfusion (Day 1). The color scale bar shows the photon counts (photon (p)/sec/cm²/sr). (B) Quantification of luminescence signals in cutaneous I/R area in OKD 48 mice. n = 4 in each group. (C) mRNA levels of oxidative stress-associated factors, Nrf2, Trx2 and HO-1 in the I/R area at 24 hours after reperfusion (Day 1). mRNA levels in control mice were assigned as values of 1. Values represent mean ± SEM. n = 4–5 mice in each group. **P < 0.01, *P < 0.05.