Figure 6.

Apelin inhibited the oxidant-induced intracellular accumulation of ROS in endothelial cells and apoptosis in fibroblasts in vitro. (A) Quantification of H₂O₂-induced (0.75 mM) intracellular ROS production in HUVECs treated with or without apelin. ROS formation in cells without treatment was assigned as a value of 1. n = 4 in each group. (B) Representative data of the amount of early apoptotic cells (Annexin V⁺, 7-AAD⁻) and necrotic cells (Annexin V⁺, 7-AAD⁺) in NIH3T3 fibroblasts with or without H₂O₂ (0.75 mM) and/or apelin. (C) Quantitation of the amount of early apoptotic cells (Annexin V⁺, 7-AAD⁻) and necrotic cells (Annexin V⁺, 7-AAD⁺) in NIH3T3 fibroblasts with or without H₂O₂ (0.75 mM) and/or apelin. Values represent means ± SEM in four independent experiments. **P < 0.01, *P < 0.05.