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. 2020 Jan 28;11:567. doi: 10.1038/s41467-019-14131-z

Fig. 6. Pt NPs reverse the DNR resistance of tumor cells.

Fig. 6

a DNR-sensitive and DNR-resistant K562 cell tumor-bearing nude mice were treated with PBS (ctrl), 0.1 mg kg−1 DNR (DNR), 1 mg kg−1 cisplatin (cisplatin), 1 mg kg−1 Pt NPs (Pt NPs), 0.1 mg kg−1 DNR combined with 1 mg kg−1 cisplatin (DNR + cisplatin) or 1 mg kg−1 DNR-Pt NPs (DNR@Pt NPs). The tumors were excised and their volumes measured. b DNR-sensitive and DNR-resistant K562 cell (left, n = 5) or HepG2 cell (right, n = 3) tumor-bearing nude mice were treated as described in panel a and the tumor volumes were measured. * P < 0.05. c TUNEL staining of K562 tumor tissue sections. The staining labeled the nuclei of apoptotic cells brown and viable cells blue. Scale bar = 50 µm. d The percentage of apoptotic cells was determined (n = 3). * P < 0.05. e Western blot analysis of the levels of cleaved caspase-3, -8, and -9 in K562 tumor lysates. β-actin was detected as a loading control. Data = Mean + /− Standard deviation.