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. 2020 Jan 28;10:1315. doi: 10.1038/s41598-020-57913-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activity of RgPAL and MEGA_A1 in calf serum. Relative amino acid concentrations are shown for different enzyme concentrations. Starting concentrations were approximately 1000 µM for L-Tyr and 200 µM for L-Phe and final concentrations were determined by reversed-phase HPLC, after incubation with enzyme. Error bars shown are based on duplicates of the [E] = 0 measurement.