Table 2.

Ranges of wzi-3 and wzi-4 melting temperatures of the most epidemiologically relevant clades and Sequence Types.

Clade	MLST profile	wzi allele	Min-Max wzi-3 T° (°C)	Min-Max wzi-4 T° (°C)	Range wzi-3 T° (°C)	Range wzi-4 T° (°C)	# of strains
ST258 Clade 2	ST512	wzi_154	83.50–84.00	83.33–83.50	0.50	0.17	23
	ST258	wzi_154	83.83–83.83	83.50–83.50	0	0	3
ST258 Clade 1	ST258	wzi_29	83.50–84.00	84.33–84.67	0.50	0.34	8
ST307	ST307	wzi_173	84.00–84.50	82.33–82.83	0.50	0.50	19
ST101	ST101	wzi_137	84.83–85.00	83.33–83.83	0.17	0.50	12
ST11	ST11	wzi_24	85.00–85.00	83.50–83.50	0	0	2
ST15	ST15	wzi_24	85.00–85.17	83.83–83.83	0.17	0	2
	ST15	wzi_89	82.33–82.33	84.00–84.00	0	0	1