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. 2020 Jan 28;11:550. doi: 10.1038/s41467-020-14312-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schema of iHGG generation. b Designs for gene editing indicating placement of sgRNAs. c Genotyping PCR and d Semi-quantitative RT-qPCR evaluating designated edits. Data are representative of three replicates, n = 3. Data are represented as mean ± SD. e RT-qPCR results of markers for iPSCs and NPCs. Data are representative of three replicates, n = 3. Data are represented as mean ± SD. f Kaplan–Meier curves showing survival of mice engrafted with (left) PTEN⁻^/− NPCs, PTEN⁻^/−;NF1⁻^/− NPCs, (right) TP53⁻^/− NPCs, and TP53⁻^/−;PDGFRA^Δ8–9 NPCs. Statistical significance was evaluated by the log-rank test. n = 4 animals for each arm for each model. Source data are provided as a Source Data file.