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. 2020 Jan 28;10:1347. doi: 10.1038/s41598-020-58177-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunoregulatory effects of BGZLS10-17 supernatants on MLNC. The effect of BGZLS10-17 supernatants on metabolic activity (MTT) (a), proliferation (b), and production of proinflammatory cytokines IL-17A (c) and IFN-γ (d) of ConA stimulated MLNC, after the 72 h treatment with 2.5% supernatant without GABA (MRS), 2.5% supernatant with 4 mM bacterial GABA (MRS/MSG), and 2.5% supernatant with addition of 4 mM artificial GABA (MRS/art. GABA). All values are presented as mean ± SD from three independent experiments. One-way ANOVA with the Dunnett’s test was used to compare multiple groups. The statistical significance of MTT and cytokine production is shown (*p < 0.05; **p < 0.01, ***p < 0.001).