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. 2020 Jan 28;10:1347. doi: 10.1038/s41598-020-58177-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BGZLS10-17 supernatants increase tolerogenic properties of MLNC. The effect of BGZLS10-17 supernatants on mRNA level of Foxp3 (a), mRNA level of TGF-β (b), and production of IL-10 (c) of ConA stimulated MLNC. MLNC were treated with 2.5% supernatant without GABA (MRS), 2.5% supernatant with 4 mM bacterial GABA (MRS/MSG), and 2.5% supernatant with addition of 4 mM artificial GABA (MRS/art. GABA). mRNA levels were measured after 24 h, while the production of IL-10 was measured after 72 h. All values are presented as mean ± SD from three independent experiments. One-way ANOVA with the Dunnett’s test was used to compare multiple groups. The statistical significance is shown (*p < 0.05; **p < 0.01, ***p < 0.001).