Figure 4.
The influence of BGZLS10-17 supernatants on autophagy of ConA stimulated different types of T cells (CD4T, CD8T, NKT, and NK). The percentage and mean of CD3+CD161−CD4+LC3+, CD3+CD161−CD4−LC3+, CD3+CD161+LC3+, CD3−CD161+LC3+ cells were assessed by flow cytometry. MLNC were treated with 2.5% supernatant without GABA (MRS), 2.5% supernatant with 4 mM bacterial GABA (MRS/MSG), and 2.5% supernatant with addition of 4 mM artificial GABA (MRS/art. GABA) for 24 h. Representative histograms from two experiments are presented.