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. 2020 Jan 28;10:1347. doi: 10.1038/s41598-020-58177-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The influence of BGZLS10-17 supernatants on the expression of pro-inflammatory molecules MHC-II and CD80 and autophagy of dendritic cells (DC). The percentage of OX62⁺ MHC-II⁺, OX62⁺ CD80⁺, and OX62⁺ LC3⁺ was assessed by flow cytometry. MLNC were treated with 2.5% supernatant without GABA (MRS), 2.5% supernatant with 4 mM bacterial GABA (MRS/MSG), and 2.5% supernatant with addition of 4 mM artificial GABA (MRS/art. GABA) for 24 h. Representative histograms from two experiments are presented.