Table 1.

Working cell concentration for hiPSC cryopreservation^a.

Cultureware	Growth surface area	Number of cells	Step 1. Cell collection		Step 2. CPA introduction		Number of cryogenic vials
			Volume of buffer	2 × cell conc.	Volume of 2 × CPA solution	Final cell conc.
6-well plate	9.5 cm2	1.5 ×106/well	500 μl/well	3 ×106/ml	500 μl/well	1.5 ×106/ml	1/well
T25 flask	25 cm2	4 ×106	1.3 ml		1.3 ml		2
T75 flask	75 cm2	1.2 ×107	3.9 ml		3.9 ml		7
T175 flask	175 cm2	2.8 ×107	9.2 ml		9.2 ml		18

^aNumerical values in this table are estimates of the respective parameters. CPA, cryoprotective agent.