Fig. 2.

Positions and sequences of the spacers used. A) Positions of spacers and associated sequences relative to the OsCAO1 gene. PAM (CAAA (TTTN PAM on complementary strand) for LbCPF1, CCA for CAS9 (TGG (NGG on complementary strand)) and AGG for BECAS9) are shown in blue, spacers are in red, and genomic sequences are in black. B) Theoretical mutation introduced by a C- > T transversion by BECAS9, which leads to the replacement of the C nucleotide of the CAG splicing site by a T nucleotide and introduces a stop codon (TAG) instead of the amino acid glutamine