Fig. 4.

Detection of porcine epidemic diarrhea virus spike (PEDV S) protein expression level in the cell lysate of S-Bm inoculated BmN cells. The BmN cells inoculated with 10 multiplicity of infection (MOI) of the S-Bm were harvested and lysed 3 days after the inoculation. The proteins in the cellular lysate were separated by protein electrophoresis, transferred onto the PVDF membrane, and probed with the anti-6× His tag antibody. a The characterization of PEDV S protein from S-Bm inoculated BmN cells. Cell only: the samples from non-inoculated BmN cells; wild-type: the samples from wild type BmNPV inoculated BmN cells; S-Bm the samples from the S-Bm inoculated BmN cells. Single star icon represented the intact S protein on the predicted molecular weight; the double star icon represented the possible molecular weight of the cleaved S protein. b The determination of the yield of the PEDV S protein from S-Bm inoculated BmN cells. S-Bm the samples from the S-Bm inoculated BmN cells, standard the standard proteins of 6× His, GAPDH control cellular protein