Fig. 1.

Gel picture showing the presence and absence of a Hopscotch Region (with all three primers Forward, Reverse and Internal Reverse); F + R only amplified in Teosinte and gave ~ 355 bp product, F + IR only amplified in Maize and gave ~ 583 bp product. b Tourist transposable element (with Forward, Reverse and Internal reverse primer); F + R produced ~ 594 bp product in Maize and ~ 186 bp product in Teosinte; F + IR not amplified in teosinte but produced ~ 383 bp product in Maize; c Gene structure of teosinte branched1 gene based on FGENESH for genotype MGUSP101 (representing tb1 gene structure in all maize genotypes) and d Gel electrophoresis showing the presence of ~ 6 bp insertion in all teosinte accessions used in study. Product size amplified was ~ 133 bp in all maize and ~ 139 bp in all teosinte