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. 2020 Jan 22;10:3114. doi: 10.3389/fimmu.2019.03114

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Copyright © 2020 Entwistle, Gregory, Oliver, Branchett, Puttur and Lloyd.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ILC2 marker expression varies between strain and treatment. (A) tSNE plot of 4,360 lung Gata3⁺ cells from both C57BL/6 and BALB/c mice. (B) Representative histograms for extracellular markers on Gata3⁺ cells taken from C57BL/6 and BALB/c mice treated with PBS, HDM, ALT, or rIL-33. Percentage of Gata3⁺ cells expressing (C) CD25, (D) CD90, (E) CD127, (F) KLRG1, (G) SCA1, and (H) ST2 and accompanying tSNE plots. Data is the combination of two individual experiments, n = 10 per group. ^†P < 0.05, ^††P < 0.01, ^†††P < 0.001, ^††††P < 0.0001 compared to PBS control. *P < 0.05, **P < 0.01, ****P < 0.0001 comparing between strains or treatment groups.