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. 2020 Jan 22;10:3114. doi: 10.3389/fimmu.2019.03114

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Copyright © 2020 Entwistle, Gregory, Oliver, Branchett, Puttur and Lloyd.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ILC2 marker expression varies with ex vivo re-stimulation. (A) tSNE plot of 4,437 lung Gata3⁺ cells from both C57BL/6 and BALB/c mice. Representative histograms for extracellular and intracellular markers on Gata3⁺ cells taken from C57BL/6 and BALB/c mice either left in media or stimulated with brefeldin A (Bref) or PMA, ionomycin and Bref (PIB). Percentage of Gata3⁺ cells expressing (B) CD25, (C) CD90, (D) CD127, (E) KLRG1, (F) SCA1, (G) ST2, (H) IL-5, and (I) IL-13 and accompanying tSNE plots. Data is the combination of two individual experiments, n = 10 per group. ^††P < 0.01, ^†††P < 0.001, ^††††P < 0.0001 compared to Media control. *P < 0.05, ***P < 0.001, ****P < 0.0001 comparing between strains or cell stimulations.