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. 2020 Jan 22;10:3114. doi: 10.3389/fimmu.2019.03114

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Copyright © 2020 Entwistle, Gregory, Oliver, Branchett, Puttur and Lloyd.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Increasing the number markers used to define ILC2s decreases their perceived frequency. Percentage of Gata3⁺ cells after subsequent gating on extracellular markers, in order of prevalence, in the lungs of C57BL/6 (A) or BALB/c mice (B). Percentage of Gata3⁺IL-13⁺ cells after subsequent gating on extracellular markers, in order of prevalence, in the lungs of C57BL/6 (C) or BALB/c mice (D). Percentage of Gata3⁺ cells in the population using sequential extracellular markers, in order of prevalence, in the lungs of C57BL/6 (E) or BALB/c mice (F).