Figure 7.

Lineage negative IL-13eGFP⁺ cells show the same extracellular marker expression as Gata3⁺ cells. (A) Contour plot depicting the GFP expression of ILC2s in WT and IL-13eGFP mice. (B) Gating schematic of Il13^gfp+ and Gata3⁺ ILC2s. Populations are pre-gated on Single cells, Live, CD45+, Lymphoid, Lineage⁻ (TCRβ, TCRγδ, CD3e, CD5, CD19, CD11b, CD11c, FCεR1, GR-1, F4/80, and TER-119 negative), CD3⁻, Nkp46⁻. (C) Total number of Lineage⁻ CD3⁻ Nkp46⁻ GFP⁺, or Gata3⁺ cells. (D) Frequency of Lineage⁻ CD3⁻ Nkp46⁻ GFP⁺, or Gata3⁺ as a proportion of CD45⁺ cells. Percentage of CD25 (E), CD90.2 (F), CD127 (G), KLRG1 (H), expressing GFP⁺ or Gata3⁺ lung cells. (F) Percentage of CD90 expressing GFP⁺ or Gata3⁺ lung cells. (G) Percentage of CD127 expressing GFP⁺ or Gata3⁺ lung cells. (H) Percentage of KLRG1 expressing GFP⁺ or Gata3⁺ lung cells. (I) Percentage of SCA1 expressing GFP⁺ or Gata3⁺ lung cells. (J) Percentage of ST2 expressing GFP⁺ or Gata3⁺ lung cells. Data is the combination of two individual experiments, n = 10 per group. *P < 0.05, **P < 0.01.