Table 1.
Exhaustion markers for TEX profiling.
| Exhaustion markers | Predicted expression vs. TN TEFF TMEM | Functional role | Minimal exhaustion panel |
|---|---|---|---|
| 2B4 | UP | Co-regulatory receptor | X |
| Amphiregulin | UP | Cytokine | |
| CCL3 | UP | Chemokine | |
| CCR7 | DN | Chemokine receptor | |
| CD38 | UP | Ectoenzyme | |
| CD39 | UP | Ectoenzyme | X |
| CD7 | UP | Co-regulatory receptor | |
| CD73 | DN | Ectoenzyme | |
| CD127 | DN | Interleukin receptor | X |
| CTLA-4 | UP | Co-regulatory receptor | X |
| CXCL10 | UP | Chemokine | |
| CXCR5 | UP | Chemokine receptor | X |
| Eomes | UP | Transcription factor | X |
| Granzyme K | UP | Cytotoxic molecule | |
| Helios | UP | Transcription factor | |
| IFN-γ | ns | Cytokine | |
| IL-2 | DN | Cytokine | |
| IL-10 | UP | Cytokine | |
| IL-21 | UP | Cytokine | |
| Lag-3 | UP | Co-regulatory receptor | |
| PD-1 | UP | Co-regulatory receptor | X |
| Ptger2 | UP | Prostaglandin receptor | |
| TCF1 | DN | Transcription factor | X |
| TIGIT | UP | Co-regulatory receptor | X |
| TNF | ns | Cytokine | X |
| TOX | UP | Transcription factor | X |
| XCL-1 | UP | Chemokine |
Markers were selected based on exhaustion-specific expression patterns using transcriptomic and epigenomic profiling and validated using mass cytometry. Markers associated with T cell exhaustion, their predicted expression on TEX compared to other cell populations, their functional role as well as their utility for a minimal exhaustion panel is noted. Moreover, cytokines required for the assessment of TEX function are included.