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. 2020 Jan 23;12:531–539. doi: 10.2147/CMAR.S232632

Figure 3.

Figure 3

BRMS1L affected the migration and invasion of ESCC via transcriptionally suppressing ITGA7 expression. (A and B) Silence of BRMS1L reduced ITGA7 expression in mRNA and protein levels. (C) Overexpression of BRMS1L increased ITGA7 expression in protein levels. (D) Luciferase reporter assay showed that silence of BRMS1L increased ITGA7 promoter activity and overexpression of BRMS1L reduced ITGA7 promoter activity. (E) ITGA7 siRNA effectively knockdowned ITGA7 mRNA level in TE-1D cells. (F–H) TE-1D cells were transfected indicated siRNAs for 48 hrs and subjected to wound healing assay (F), migration assay (G) and invasion assay (H). (I) TE-1D cells were transfected with indicated siRNAs and EMT markers E-Cadherin, N-Cadherin, Snail and Slug mRNA level were examined. n=3, *P < 0.05, **P < 0.01, compared to NC/Vector.