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Characteristics of CNPs. (A) Visible mineralized biofilms on the right bottle after 6 weeks; (B) the white precipitate after centrifugation; (C) TEM showed that CNPs had irregularly spherical morphology with a dense, needle-like hydroxyapatite shell; (D) Gram staining of CNPs; (E) Alizarin red staining of CNPs; (F) von Kossa staining of CNPs. CNPs, calcifying nanoparticles; TEM, transmission electron microscope.
