FIG. 7.
Inhibition of RyRs with tetracaine blocked hypoxic ROS generation in PASMCs and mitochondria. ROS generation, measured using H2DCF/DA (DCF) (A), lucigenin (B), and Red CC-1 (C), in PASMCs treated without and with tetracaine (10 μM) for 5 min following normoxia or hypoxia for 5 min. ROS generation, evaluated using DCF (D), lucigenin (E), and Red CC-1 (F), in isolated mitochondria from PASMCs untreated and treated with tetracaine (10 μM) for 5 min followed by normoxia or hypoxia for 5 min. Data were obtained from three separate experiments. *p < 0.05 compared with normoxic control, and #p < 0.05 compared with hypoxic control. RyR, ryanodine receptor.