FIG. 1.

EGFP silencing and siRNA accumulation in H1299, HeLa, HEK293, and HepG2 cells. Cells were transfected with 100 nM fluorescently labeled siRNA and 2.3 μg/mL LF2K and assayed 24 h post-transfection using flow cytometry (10,000 events). (A) Control value = 0 and (B) control value = 1. In each panel, values for each cell line were statistically different from all others, P < 0.05; error bars represent ±1 standard deviation; n = 4. Statistical analysis was performed using one-way ANOVA, followed by Tukey's HSD post hoc analysis. ANOVA, analysis of variance; LF2K, Lipofectamine 2000; siRNA, small interfering RNA.