FIG. 3.

Influence of endocytic protein overexpression on the intracellular accumulation of siRNAs. Shown is the relative fluorescence of complexes containing fluorescently labeled siRNAs delivered to cells transiently expressing EGFP-labeled endocytic proteins (A H1299; B HEK293; C HeLa; D HepG2). Plasmid transfection did not alter siRNA accumulation. Cells were assayed 4 h post-transfection using flow cytometry (10,000 events). Error bars represent ±1 standard deviation; n = 3. Statistical analysis was performed using one-way ANOVA, followed by Tukey's HSD post hoc analysis. *Significant difference (P < 0.05) compared to siRNA accumulation in cells transfected with EGFP-only plasmid.