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. 2020 Jan 28;30(1):22–32. doi: 10.1089/nat.2019.0804

FIG. 4.

FIG. 4.

Influence of endocytic inhibitors on EGFP silencing and siRNA accumulation in co-cultured and monocultured populations. Co-cultured populations consisted of H1299, HEK293, HeLa, and HepG2 cells. EGFP-expressing cells (A H1299; B HEK293; C HeLa; D HepG2) were pretreated with endocytic inhibitors and assayed 24 h post-transfection using flow cytometry (5,000 events). x-Axis: −100% (inhibited silencing) versus 100% (enhanced silencing). y-Axis: −100% (inhibited siRNA accumulation) versus 100% (enhanced siRNA accumulation). Error bars represent ±1 standard deviation; n = 3. Statistical analysis was performed using one-way ANOVA, followed by Tukey's HSD post hoc analysis. *Significant difference (P < 0.05) compared to delivery in the absence of an inhibitor.