Figure 4.

Confocal laser scanning microscopy images of P. aeruginosa biofilms before CPC-quatsome treatment (a), after 0.05% CPC-quatsome treatment for 5 min (b), and after 0.05% CPC-quatsome treatment for 2 h (c). Quatsome are stained red (DiI), live P. aeruginosa green (SYTO-9), dead P. aeruginosa blue (SYTOX). The large center images showed the X-Y view of the central layer from the z-stack; the top bars showed the X-Z view of the central layer from the y-stack, the vertical bars to the right showed the Y-Z view of the central layer from the x-stack for the same biofilm. (A color version of this figure is available in the online journal.)