Figure 2.

Labeling conditions of acceptor erythrocytes with CTFR. Erythrocytes were labeled using different concentrations of CTFR diluted in different solvents – 1× PBS (a–b) or RPMI 1640 (c–d) – at different hematocrits – 4% (a and c) or 8% (b and d) – and incubated at 37°C for 2 h in a shaking incubator. Labeled erythrocytes were further mixed with unlabeled erythrocytes and analyzed by flow cytometry.