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. Author manuscript; available in PMC: 2020 Jan 29.
Published in final edited form as: Methods Enzymol. 2019 Jan 3;619:145–159. doi: 10.1016/bs.mie.2018.11.006

Table 1:

Concentrations of key reagents used during extract preparation and crosslinking

Reagent Stock concentration Final concentration
For spheroplasting
DTT 1 M 30 mM
Zymolyase 20T 20 mg/mL 0.8 mg/mL
 
For cell lysis
ATP 100 mM 2 mM
ATPγS 100 mM 2 mM
AMP-PNP 100 mM 2 mM
 
For crosslinking
CuCl2 10 mM 250 μM
NEM 200 mM 10 mM
EDTA 100 mM 10 mM
DTT (reduced control only) 1 M 20 mM