Key Resources Table.
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Mouse anti-B-tubulin MAb E7 | Developmental Studies Hybridoma Bank | RRID:AB_528499 |
| Mouse anti-phospho-SAPK/JNK (Thr183/Tyr185) MAb G9 | Cell Signalling Technology | RRID:AB_2307321 |
| Rabbit anti-dFOXO | Michael Marr | BRD10 |
| Cy3-conjugated AffiniPure goat anti-rabbit antibody | Jackson ImmunoResearch | Cat. no. 111-167-003 |
| FITC-conjugated AffiniPure donkey anti-mouse antibody | Jackson ImmunoResearch | Cat. no. 715-095-151 |
| Anti-rabbit HRP-conjugated IgG (H+L) | GE Healthcare | |
| Anti-mouse HRP-conjugated IgG (H+L) | GE Healthcare | |
| Rat monoclonal anti-HA-tag [7C9] | ChromoTek | |
| Deposited data | ||
| Agilent microarray data | This article | Array express: E-MTAB-6253 |
| Experimental models: organisms/strains | ||
| Drosophila melanogaster: Schneider 2, CVCL_Z232 | Invitrogen | RRID: CVCL_Z232 |
| Homo sapiens: HeLa CCL-2 | American Type Culture Collection (ATCC) | RRID: CVCL_0030 |
| D. melanogaster: hopTum | Bloomington Drosophila Stock Center | RRID: BDSC_8492 |
| D. melanogaster: w1118 | Bloomington Drosophila Stock Center | RRID: BDSC_5905 |
| D. melanogaster: w1118; foxoΔ94/TM6B, Tb1 | Bloomington Drosophila Stock Center | RRID: BDSC_42220 |
| D. melanogaster: w1118 P{w+mC=UAS-bsk.DN}2 | Bloomington Drosophila Stock Center | RRID: BDSC_6409 |
| D. melanogaster: bsk1 cn1 bw1 sp1/CyO, | Bloomington Drosophila Stock Center | RRID: BDSC_3088 |
| D. melanogaster: w*; cno3 P{ry+t7.2=A92}pucE69/TM6B, P{w+mC=iab-2(1.7)lacZ}6B, Tb1 | Bloomington Drosophila Stock Center | RRID: DGGR_109029 |
| D. melanogaster: w1118; P{GD10555}v3413 UAS-bskRNAi | Bloomington Drosophila Stock Center | RRID:FlyBase_FBst0460476 |
| D. melanogaster: w1118; P{GAL4}fat (FB-GAL4) | Gronke et al (2003) | RRID:FlyBase_FBti0013267 |
| D. melanogaster: w1118; P{GD10555}v3413 UAS-bskRNAi | Bloomington Drosophila Stock Center | RRID:FlyBase_FBst0460476 |
| D. melanogaster: y1 w67c23; P{EPgy2}PxtEY03052 | Bloomington Drosophila Stock Center | RRID:BDSC_15620 |
| D. melanogaster: PBac{WH}Pxtf05258 | The Exelixis Collection at the Harvard Medical School | RRID: FBti0067872 |
| D. melanogaster: dfoxo-v3-mCherry | Kakanj et al (2016) | N/A |
| Beauveria bassiana | CABI | (T. Petch, 1947) IMI: 12942 |
| Oligonucleotides | ||
| foxo-ChIP-control forward primer (5′-AGCCCGCGAAGATACAAGAG-3′) | This article | N/A |
| foxo-ChIP-control reverse primer (5′-AGCCACAAACAGCGACAGAA-3′) | This article | N/A |
| pdcd4-ChIP forward primer (5′-CCGTTGGGAGTCTCTCTCTC-3′) | This article | N/A |
| pdcd4-ChIP reverse primer (5′-GCTCTCCCCACCTTCTCACC-3′) | This article | N/A |
| hsp26-ChIP forward primer (5′-GTGCGCCTGTATGAGTGAGA-3′) | This article | N/A |
| hsp26-ChIP reverse primer (5′-GTGGGAGATTGCTGGCGTTA-3′) | This article | N/A |
| 4EBP-ChIP forward primer (5′-CAAGAACCAGCCGGTTTGTC-3′) | This article | N/A |
| 4EBP-ChIP reverse primer (5′-GCTCTCTTCTCGCTCTTTCG-3′) | This article | N/A |
| Pxt-RT-PCR forward primer (5′-GCAGCTCCTCGATGTGATTGAAAC-3′) | This article | N/A |
| Pxt-RT-PCR reverse primer (5′-CTAGAGTGCGAGCGAGAGGTAAGA-3′) | This article | N/A |
| RpL32-RT-PCR forward primer (5′-ATCCGCCACCAGTCGCATCGATATGCTAAG-3′) | This article | N/A |
| RpL32-RT-PCR reverse primer (5′-TCTTGAGAACGCAGGCGACCGTTGGGGTTG-3′) | This article | N/A |
| DptA-RT-PCR forward primer (5′-GCAGTTCACCATTGCCGTCGCCTTACTTTG-3′) | This article | N/A |
| DptA-RT-PCR reverse primer (5′-TGAAGATTGAGTGGGTACTGCGGTGGTGGA-3′) | This article | N/A |
| Drs-RT-PCR forward primer (5′-ATCAAGTACTTGTTCGCCCTCTTCGCTGTC-3′) | This article | N/A |
| Drs-RT-PCR reverse primer (5′-CTCGTTGTCCCAGACGGCACAGGGACCCTT-3′) | This article | N/A |
| Recombinant DNA | ||
| Plasmid: pTRE3G | Clontech | 631173 |
| pTRE3G-3XFLAG | This article | N/A |
| pTRE3G-3XFLAG-Pxn | This article | N/A |
| Software and algorithms | ||
| FIJI | Schindelin et al (2012) | https://imagej.net/Fiji |
| Marray | Paquet (2009) | https://bioconductor.org/packages/release/bioc/html/marray.html |
| Limma | Ritchie et al (2015) | http://bioconductor.org/packages/release/bioc/html/limma.html |
| Bioconductor version 3.4 | http://bioconductor.org | https://bioconductor.org/news/bioc_3_4_release/ |
| R version 3.1.2 | https://www.R-project.org | https://cran.r-project.org/bin/windows/base/old/3.1.2/ |
| ClueGo | (Bindea et al, 2009) | https://apps.cytoscape.org/apps/cluego |
| esyN | (Bean et al, 2014) | http://www.esyn.org |
| Cytoscape | (Shannon et al, 2003) | https://cytoscape.org |
| iRegulon | (Janky et al, 2014) | http://iregulon.aertslab.org |