Figure 3.

Normal Magnitude and Output of the GC Response in Cxcr5^fl/flFoxp3^cre-ERT2 Mice

Analysis of the GC response 14 days after s.c. immunization with NP-KLH/alum.

(A) Flow cytometry contour plots of Bcl6⁺Ki67⁺B220⁺ GC B cells.

(B and C) Quantification of the (B) frequency and (C) absolute number of Bcl6⁺Ki67⁺ B cells.

(D) Quantification of confocal images of the average GC area per mouse of the indicated genotypes.

(E) Flow cytometry contour plots of Tfh cells, gated as CXCR5⁺PD-1⁺ of Foxp3⁻CD4⁺ cells.

(F and G) Quantification of the (F) percentage and (G) number of CXCR5⁺ Tfh cells.

(H) Quantification of confocal images of the average number of CD3⁺Foxp3⁻ Tfh cells per GC area per mouse of the indicated genotypes.

(I) Levels of anti-NP7 antibodies of the indicated isotypes in the sera.

(J) Levels of anti-NP20 antibodies of the indicated isotypes in the sera.

(K) Ratio of NP20/NP7 of the indicated isotypes in the sera.

(L) Serum levels of IgG specific for dsDNA.

Each symbol represents one mouse, the horizontal bars represent mean values, and the error bars show the SD. The p values were determined using a Mann-Whitney U test. For the quantification of confocal images, 4–10 GCs were imaged per mouse. Data represent two independent experiments.