Figure 3.

Loss of TbATR Impairs Control of VSG Expression Site Transcription

(A) qRT-PCR of VSGs within 4 silent BES are shown (24 and 36 h post-RNAi, +) as fold-change in level relative to uninduced cells (−; set at 1); data are shown for clones CL1 and CL2, and error bars denote ± SEM.

(B) Heatmaps of differentially expressed BES and MES VSG transcripts, plotted as log₂fold change in +RNAi relative to −RNAi.

(C and D) RNA-seq read depth across the active BES (BES1; C) and one silent BES (BES3; D) after 24 and 36 h of growth with (T+) or without (T−) RNAi; data from three replicates are overlaid (pink, blue, and orange). ESAG6 and ESAG7 genes are shown in green, other ESAGs in white, and VSG in orange. The boxed graphic shows a simplified layout of a BES (telo, telomere; arrow, promoter).