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. 2020 Jan 21;30(3):836–851.e5. doi: 10.1016/j.celrep.2019.12.049

Figure 6.

Figure 6

TbATR Loss Results in the Accumulation of VSG-Associated Damage

(A) γH2A ChIP-seq enrichment across the active BES (BES1, VSG2) and a silent BES (BES3, VSG6) after 24 (orange) and 36 h (blue) of growth with RNA induction (+). γH2A ChIP-seq signal enrichment (y axis) is shown as a ratio of reads in RNAi-induced samples relative to uninduced samples (each normalized to cognate input sample). VSG is shown as a red box, ESAG6 and ESAG7 as green boxes, and other ESAGs as white boxes.

(B) Enrichment of γH2A in RNAi-induced cells relative to uninduced across the 70-bp repeats (purple box) in the active BES1 and in three silent BESs (3, 5, and 7).

(C) Metaplots showing γH2A signal enrichment after RNAi across all silent BESs, silent MESs, subtelomeric VSGs, and core VSGs (in each, VSGs are scaled to 500 bp and regions up- and downstream are plotted).

(D) γH2A signal enrichment after RNAi in the active BES1 and two silent BES (5 and 7) across the region extending from the end of the VSG (red) to the telomere (arrow, telo); the inset shows a metaplot of γH2A signal for all of the silent BESs across the same region.