Figure 3.

Effect of high salt (HS), l‐buthionine‐sulfoximine (BSO), and resveratrol (R) on renal lysosomal enzymes. Vehicle (V) animals were kept on tap water. A, Glucocerebrosidase and (B through D) cathepsin B and D activities were measured in renal proximal tubules at the end of 6‐wk treatment. Glucocerebrosidase activity was measured by using substrate 4‐methylumbelliferyl‐β‐D‐glucopyrooside. Cathepsin B and D were assayed with commercially available kits and reported as relative fluorescence units (RFU). The specificity of cathepsin B and D were assessed by inhibitors (I) provided with the kit. Experiments were performed in triplicate (n=6–8 rats). *P<0.05 vs V by 1‐way ANOVA followed by a post hoc Newman–Keuls test.