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. 2019 Dec 27;9(1):e014103. doi: 10.1161/JAHA.119.014103

Figure 3.

Figure 3

YAP (Yes‐associated protein) was involved in Sp‐1 (specificity protein 1)‐mediated SMC phenotypic modulation. A and B, Platelet‐derived growth factor‐BB (PDGF‐BB, 20 ng/mL for 24 h) induced YAP protein (A) and mRNA (B) expression in parallel with Sp‐1 expression. Additional siSp‐1 transfected or Mithramycin A (MTA) treatment could reverse this process. *P<0.05 vs control group. n=3. # P<0.05 vs PDGF‐BB treatment group. n=3. C and D, Plasmid‐induced Sp‐1 overexpression increased YAP gene expression at both the protein (C) and mRNA (D) level. *P<0.05 vs vector‐treated group. n=3. E and F, SMCs were transduced with control lentivirus or lentivirus encoding wild‐type or 1S mutant YAP, and treated with MTA (20 nmol/L) for 48 h. Sp‐1 inhibitor MTA regulated SMC phenotypic modulation and cyclin D expression could be partially reversed by overexpression of YAP. *P<0.05 vs MTA‐treated group. n=3. G, Cell number was counted at the time points as indicated to determine the effects of MTA, MTA+YAP, and MTA+YAP 1S on proliferation. *P<0.05 YAP vs MTA‐treated group. n=3. # P<0.05 YAP 1S vs MTA‐treated group. n=3. SMMHC indicates SM myosin heavy chain.