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. 2020 Jan 4;9(1):e013452. doi: 10.1161/JAHA.119.013452

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© 2020 The Authors. Published on behalf of the American Heart Association, Inc., by Wiley.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Human CardioChimeras (hCCs) are created from c‐kit⁺ cardiac interstitial cell (cCIC) and mesenchymal stem cell (MSC) fusion. A, Fusion‐suspension of cCICs and MSCs. Initial fused cells undergo a mitotic event to combine chromatin content followed by sorting of successfully fused cells (hCC). B, Intraclass correlation of the hCCs for GFP (green fluorescent protein) and mCherry. C, Immunoblot analysis of cCIC‐GFP, MSC‐mCherry, nontransduced (NT) MSC, and hCCs. GAPDH is used as the loading control. DAPI indicates 4′,6‐diamidino‐2‐phenylindole; PL, protein ladder.