Table 1.
Primers used in this study
| Primer ID | Used for | Primer sequence (5ʹ–3ʹ) |
|---|---|---|
| Hc-hrg-2-F | cDNA and genomic DNA | ATGATTCTCTTGGTTTCTGTTGCTG |
| Hc-hrg-2-R | cDNA and genomic DNA | AGCAAACTCTTTTCCAAAAACCGTATCTCG |
| PE-Hc-hrg-2-F | Prokaryotic expression | CGGGATCCATGATTCTCTTGGTTTCTGTTGCTG |
| PE-Hc-hrg-2-R | Prokaryotic expression | CCCAAGCTTTCATTCTTCAGCAAACTCTTTTCCAAAAA |
| RA-Hc-hrg-2-R1 | 3ʹ RACE | CCTAAATTACCTGTCCCACTCCC |
| RA-Hc-hrg-2-R2 | 3ʹ RACE | GTTAAAACGAGCGTGAGGCCAT |
| real-Hc-hrg-2-F | real-time PCR | ACTGCCCATAGTGCTTCCAC |
| real-Hc-hrg-2-R | real-time PCR | AGTCCTCGACAGGGAACTGA |
| Real-Hc-actin-1-F | real-time PCR | TCAATTGTCGGACGTCCTCG |
| Real-Hc-actin-1-R | real-time PCR | AGGGGAGCTTCGGTCAAAAG |
| EE-Hc-hrg-2-F | Eukaryotic expression | CGGAATTCATGATTCTCTTGGTTTCTGTTGCTG |
| EE-Hc-hrg-2-R | Eukaryotic expression | CGGGATCCTCATTCTTCAGCAAACTCTTTTCCAAAAA |
| EE-Hc-hrg-2(ΔTMD)-F | Eukaryotic expression | CGGAATTCATGATTCTCAAGAAGGACAAGAAAGAGGTT |
| EE-Hc-hrg-2(ΔTMD)-R | Eukaryotic expression | CGGGATCCTCATTCTTCAGCAAACTCTTTTCCA |
| EE-Hc-hrg-2(ΔGST-N)-F1 | Eukaryotic expression | CGGAATTCATGATTCTCTTGGTTTCTGTTGCTG |
| EE-Hc-hrg-2(ΔGST-N)-R1 | Eukaryotic expression | CGACGCGAGAGGCTTCACGGTATCGGATTTCCA |
| EE-Hc-hrg-2(ΔGST-N)-F2 | Eukaryotic expression | GTGAAGCCTCTCGCGTCGTCTA |
| EE-Hc-hrg-2(ΔGST-N)-R2 | Eukaryotic expression | CGGGATCCTCATTCTTCAGCAAACTCTTTTCCA |
| EE-Hc-hrg-2(ΔGST-C)-F1 | Eukaryotic expression | CGGAATTCATGATTCTCTTGGTTTCTGTTGCTGTCTGG |
| EE-Hc-hrg-2(ΔGST-C)-R1 | Eukaryotic expression | TTTTCCAAAAACCGTATCCTTGAAGTCCTCGAC |
| EE-Hc-hrg-2(ΔGST-C)-R2 | Eukaryotic expression | CGGGATCCTCATTCTTCAGCAAACTCTTTTCCAAAAAC |
| EE-Hc-hrg-2(TMD)-F | Eukaryotic expression | CGACCGGTCGCCACCATGGTGAGCAAGGG |
| EE-Hc-hrg-2(TMD)-R1 | Eukaryotic expression | AACGACAGCCAACCAGACAGCAACAGAAACCAAGAAGCTTGAGCTCGAGATCTT |
| EE-Hc-hrg-2(TMD)-R2 | Eukaryotic expression | CGGAATTCCTACAAGAAAACAAAATATGCGACGATACTAACGACAGCCAACCAGACAG |
Note: Restriction sites are underlined