Fig. 2.

TRIM6 knockdown inhibited CRC cell proliferation and induced G2/M arrest. a, The protein level of TRIM6 in human normal colorectal mucosa cell line (FHC) and CRC cell lines (LOVO, Sw620, Sw1116, HCT-8 and HCT116) was examined by western blotting. GAPDH was used as a loading control. b, Knocking down of TRIM6 in HCT-8 and HCT116 cells. Cells were infected with lentivirus expressing TRIM6 shRNA (shTRIM6–1, − 2 and − 3) or control shRNA (shNC). At 48 h post infection, protein was extracted and TRIM6 expression was examined by western blotting. C-F, The effects of TRIM6 on the proliferation, cell cycle distribution as well as expression of Cyclin B1 and c-Myc in HCT-8 and HCT116 cells were measured by CCK-8 (c), BrdU (d), flow cytometry analyses (e), and western blotting (f), respectively. *P < 0.05, **P < 0.01, ***P < 0.001 vs shNC