Fig. 3.

TRIM6 knockdown potentiated the anti-proliferative effects of 5-fluorouracil and oxaliplatin a, HCT-8 and HCT116 cells were infected with shTRIM6–1 or shNC, and treated with 40, 60, 80 or 100 μM L-OHP for 24 h. Cell proliferation was determined by CCK-8 assay, and IC50 was calculated. b, HCT-8 and HCT116 cells were infected with shTRIM6–1 or shNC, and treated with 300, 400, 500 or 600 μM 5-FU for 24 h. Cell proliferation was determined by CCK-8 assay, and IC50 was calculated. C-F, HCT-8 and HCT116 cells were infected with shTRIM6–1 or shNC, and treated with 64 μM L-OHP, 400 μM 5-FU or vehicle (DMSO) for 24 h. Cell apoptosis (c, d) and expression of cleaved-caspase3 (C-Casp3, e, f) was determined. ***P < 0.001