Fig. 1.

a Expression analysis of NFкB (RelA) and FAT1 in GBM samples. mRNA expression of NFкB (RelA) and FAT1 genes was analyzed in GBM tumors (n = 16) with respect to the levels found in human normal brain total RNA (Clontech, USA) using qPCR. Increased expression of FAT1 was found in 9 out of 16 GBM samples as compared to the normal brain. High-NFkB expressors are represented as blue dots and low-NFkB expressors as black dots. FAT1 has been depicted with grey dots. b Correlation analysis of NFкB (RelA) and FAT1 expression in GBM samples. mRNA fold expression values of NFкB (RelA) and FAT1 found in the sixteen GBM tumors were correlated using Spearman’s analysis by SPSS 11.5. A significant positive correlation was observed between NFкB (RelA) and FAT1 (r = 0.7, p = 0.002) in the studied samples. GBM samples have been displayed as high NFкB (RelA) expressors (blue dots) and low NFкB (RelA) expressors (black dots) on the basis of a cut-off value of ≥1.5 fold expression. c Correlation of NFкB (RelA) and FAT1 expression in Rembrandt GBM database. Expression values of NFкB (RelA) and FAT1 were obtained and correlated in 214 GBM cases belonging to Rembrandt database. A significant positive correlation was observed between NFкB (RelA) and FAT1 (Spearman’s ‘r’ = 0.276, p = 0.00004) in the analyzed cases. d Correlation of NFкB (RelA) and FAT1 expression in TCGA glioma database. Expression values of NFкB (RelA) and FAT1 were obtained and correlated in 153 glioma cases belonging to TCGA database. A significant positive correlation was observed between NFкB (RelA) and FAT1 (Spearman’s ‘r’ = 0.213, p = 0.008) in the analyzed cases