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. 2020 Jan 28;20:14. doi: 10.1186/s12862-020-1580-3

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Characteristics of small RNAs sequenced in 10 GDL strains. a Length distribution of small RNAs that are mapped to the reference genome and TE sequences, the known miRNAs, tRNAs, rRNAs, ncRNAs and miscRNAs were removed. b Barplots of the fractions of the first nucleotide of piRNAs in 10 GDL strains. c Pie chart of the genomic locations for all mapped piRNAs. d Pie chart of the genomic locations for the uniquely mapped piRNAs. e The ping-pong signature generated between the sense and antisense piRNA reads. The x-axis shows the nucleotides that are overlapping between a sense and antisense piRNA. The y-axis is the Z-score of the overlapping length among all the possible overlapping combinations. f Heatmap showing the RPKM values of weighted piRNAs on TEs in 10 GDL strains. Only the top 40 TEs with the highest RPKMs are shown. g Heatmap showing the RPKM values of weighted piRNAs on piRNA clusters in 10 GDL strains. Only the top 40 piRNA clusters with the highest RPKMs are shown