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. 2020 Jan 29;19:50. doi: 10.1186/s12936-020-3127-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Limit of detection of varATS qPCR determined by probit analysis. Based on a serial dilution of WHO standard material using either 3 µl of DNA supplemented by one negative DBS punch (representing LOD using 1 punch per DBS sample, light blue) or 15 µl of DNA supplemented with five negative DBS punches (representing LOD using 5 punches per DBS sample, dark blue)