Table 2.

Opsin key residues

Species	Ecology	Gene	Key tuning amino acid site in round goby					Max. spectral sensitivity (wavelength)	Reference
			180	197	277	285	308
		Bovine rhodopsin equivalent site:	164	181	261	269	292
Boleophthalmus pectinirostris	Terrestrial mudskipper	LWS1	A	H	Y	T	A	553 nm	[28]
		LWS2	A	H	F	A	A	531 nm
Periophthalmus magnuspinnatus	Terrestrial mudskipper	LWS1	S	H	Y	T	A	560 nm
		LWS2	A	H	F	T	A	546 nm
Neogobius melanostomus	Freshwater temperate rivers and lakes	LWS1	S	H	Y	T	A	560 nm	This study
		LWS2	S	H	F	T	A	550 nm*	This study

*Predicted by the key tuning sites, and Y261F shift of 10 nm; Yokoyama, 2008

Amino acid analysis of key tuning sites in Gobiidae red opsins proteins