Figure 5.

βC1 protein expressed via a PVX vector compromises resistance against TYLCV in Ty‐1 transgenic Nicotiana benthamiana. (A) Wild‐type (WT) and Ty‐1 transgenic N. benthamiana plants were infected with TYLCV and at 10 days post‐inoculation (dpi) were challenged with PVX expressing TYLCCNB‐βC1, TYLCCNB‐βC1 mutant, AYVB‐ βC1 or empty PVX vector. Eight days post‐PVX infection, pictures were taken and plants were scored for disease symptoms, indicated in the bottom right corner. (B) From the plants described under 5a, samples of systemically infected leaves were collected, DNA was isolated and the titre of TYLCV was determined via quantitative PCR. The viral titre was normalized to the presence of genomic DNA (25S rRNA) and the fold difference was calculated according to the 2^−ΔΔCt method relative to WT plant infected with TYLCV only (set to 1). Every point represents one plant. Lines represent means and standard error of the mean of biological replicates. Note that the y‐axis is split to show the low titres. Asterisks indicate significant differences according to one‐way analysis of variance. In the analysis WT TYLCV‐only infected plants were compared with the different WT co‐infected groups and the same was done for the Ty‐1 transformants (**P < 0.01; ***P < 0.001).