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. Author manuscript; available in PMC: 2020 Jan 29.
Published in final edited form as: Clin Oncol Res. 2019 Oct 10;2(5):10.31487/j.cor.2019.05.05. doi: 10.31487/j.cor.2019.05.05

Figure 5.

Figure 5.

B7-H3 expression is regulated by Treg cell cytokines. (A) Flow cytometry analysis of GEC cells stained for B7-H3 after exposure to 5 ng/mL IL-10 for 24 h showed increased expression in a representative histogram where the solid peak is the isotype control (B) Flow cytometry was done to measure B7-H3 expression on GECs after treating the cells with TGF-β (3 ng/ml) for 24 h. (C) Flow cytometry was done to measure B7-H3 expression on GECs treated with either IL-10 (5 ng/mL) or TGF-β (3 ng/ml) or both IL-10 and TGF-β. The data were expressed as the percentage of positive cells. The means ± SD are shown as the results of duplicates of one of four representative experiments: n=8, * P < 0.05, ** P < 0.01 and *** P < 0.001.