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. 2020 Jan 19;26:e919241-1–e919241-12. doi: 10.12659/MSM.919241

Figure 4.

Figure 4

KAT7 enhanced cell proliferation, cell migration, colony formation, and epithelial-mesenchymal transition (EMT) in human hepatocellular carcinoma (HCC) cell lines, Huh7 and HepG2. (A) KAT7 mRNA expression was detected using quantitative reverse transcription-polymerase chain reaction (RT-qPCR) in human HCC cell lines, Huh7 and HepG2. (B) The MTT assay evaluated the cell viabilities of HCC cells. (C) The colony formation assay was performed to assess colony formation in human HCC cell lines. (D) Transwell migration and invasion experiments were performed to evaluate human HCC cell lines. (E) RT-qPCR evaluated the mRNA levels of EMT markers for E-cadherin and vimentin. (F) Western blot was used to evaluate the expression levels of EMT markers, E-cadherin and vimentin. Studies were performed in triplicate. (* p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001).